Dynamic ASXL1 Exon Skipping and Alternative Circular Splicing in Single Human Cells.

Dynamic ASXL1 Exon Skipping and Alternative Circular Splicing in Single Human Cells.

Blog Article

Circular RNAs comprise a poorly understood new class of noncoding RNA.In this study, we used a combination of targeted deletion, high-resolution splicing detection, and single-cell sequencing to deeply probe ASXL1 circular splicing.We found that efficient circular splicing required the canonical transcriptional start site and inverted AluSx elements.Sequencing-based interrogation of isoforms after ASXL1 overexpression identified promiscuous linear splicing between all exons, with the two most abundant non-canonical 3m speedglas 9002nc linear products skipping the exons that produced the circular isoforms.

Single-cell sequencing revealed a strong preference for either the linear click here or circular ASXL1 isoforms in each cell, and found the predominant exon skipping product is frequently co-expressed with its reciprocal circular isoform.Finally, absolute quantification of ASXL1 isoforms confirmed our findings and suggests that standard methods overestimate circRNA abundance.Taken together, these data reveal a dynamic new view of circRNA genesis, providing additional framework for studying their roles in cellular biology.